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Specific blaCTX-M-8/IncI1 Plasmid Transfer among Genetically Diverse Escherichia coli Isolates between Humans and

Chihiro Norizuki1, Jun-Ichi Wachino2, Masahiro Suzuki3

  • 1Department of Pathophysiological Laboratory Sciences, Nagoya University Graduate School of Medicine, Nagoya, Japan.

Antimicrobial Agents and Chemotherapy
|April 12, 2017
PubMed

Insights

The blaCTX-M-8 gene, often found on IncI1 plasmids, was detected in Escherichia coli from humans and chickens. A specific plasmid lineage (pST113) suggests local gene transfer between these sources.

Area of Science:

  • Microbiology
  • Genetics
  • Antimicrobial Resistance

Background:

  • The blaCTX-M-8 gene is a significant cause of antimicrobial resistance in Escherichia coli.
  • Understanding the genetic elements and transmission routes of blaCTX-M-8 is crucial for public health.

Purpose of the Study:

  • To investigate the genetic characteristics of blaCTX-M-8-positive Escherichia coli isolates from human and chicken sources.
  • To determine the plasmid types and genetic lineages associated with blaCTX-M-8.

Main Methods:

  • Genomic analysis of 14 blaCTX-M-8-positive Escherichia coli isolates.
  • Plasmid typing and characterization of blaCTX-M-8-carrying plasmids.
  • Comparison of genetic contexts of blaCTX-M-8 on IncI1 plasmids.

Main Results:

  • All 14 isolates harbored blaCTX-M-8 on IncI1 plasmids.
  • Nine out of 14 isolates belonged to a specific lineage, plasmid sequence type 113 (pST113).
  • The genetic contexts of blaCTX-M-8/IncI1 pST113 plasmids were conserved across different sources.

Conclusions:

  • The findings suggest a probable local transfer of blaCTX-M-8-carrying IncI1 plasmids between human and chicken populations.
  • The prevalence of the pST113 lineage highlights its potential role in the dissemination of blaCTX-M-8.
Keywords:
Escherichia coliIncI1 plasmidblaCTX-M-8humanretail chicken meat

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