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A method for estimating the concentration of spermatozoa in the rat cauda epididymidis
W G Kempinas1, T L Lamano-Carvalho
1Department of Stomatology, Faculty of Dentistry of Ribeirão Preto, SP, Brazil.
Laboratory Animals
|April 1, 1988
Abstract:
A method for estimating the concentration of spermatozoa in the rat cauda epididymidis is described. Treatment of a sperm suspension with 0.05% collagenase for 20-60 min or 0.025% trypsin for 1-2 min at 34-37 degrees C was found to result in consistently homogeneous sperm. Sperm concentration ranged from 152.5 to 230.0 X 10(7) spermatozoa/ml, with a mean of 187.7 (+/- 5.6 SEM) X 10(7) spermatozoa/ml.