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Efficiency of Leukocyte Differential Using Flow Cytometry with CytoDiff in Different Workflows
Clinical Laboratory
|April 12, 2017
Summary
This study established review criteria for flow cytometry with CytoDiff (FCC) to assess white blood cell (WBC) differential workflows. The combination of complete blood count (CBC) after FCC and microscopy demonstrated high sensitivity and accuracy.
Area of Science:
- Clinical Hematology
- Laboratory Medicine
- Diagnostic Cytometry
Background:
- Flow cytometry with CytoDiff (FCC) is a developing method for white blood cell (WBC) differential analysis.
- Evaluating different laboratory workflows integrating FCC is crucial for optimizing diagnostic efficiency.
Purpose of the Study:
- To establish review criteria for FCC in WBC differential analysis.
- To compare the efficiency of various workflows combining FCC with hematology analyzers and microscopy.
Main Methods:
- Analysis of leukocyte differentials from 995 clinical and 278 healthy donor specimens.
- Comparison of results obtained from a hematology analyzer, FCC, and microscopy.
- Development of receiver operating characteristic (ROC) curves to define FCC thresholds for specific cell types.
Main Results:
- Good correlations were observed between methods for neutrophils, lymphocytes, and monocytes.
- Lower correlations were noted for basophils and eosinophils.
- The workflow of complete blood count (CBC) after FCC, followed by microscopy, yielded the highest sensitivity (97.07%) and accuracy (80.3%) with a low false negative rate (2.93%).
Conclusions:
- FCC can be effectively integrated into routine WBC differential workflows.
- The study provides evidence for selecting optimal hematology workflows based on FCC integration.
- The defined review criteria and workflow efficiencies offer valuable insights for laboratory practice.
