C₃ -C₄ intermediates may be of hybrid origin - a reminder

The currently favoured model of the evolution of C₄ photosynthesis relies heavily on the interpretation of the broad phenotypic range of naturally growing C₃ -C₄ intermediates as proxies for evolutionary intermediate steps. On the other hand, C₃ -C₄ intermediates had earlier been interpreted as hybrids or hybrid derivates. By first comparing experimentally generated with naturally growing C₃ -C₄ intermediates, and second summarising either direct or circumstantial evidence for hybridisation in lineages comprising C₃ , C₄ and C₃ -C₄ intermediates, we conclude that a possible hybrid origin of C₃ -C₄ intermediates deserves careful examination. While we acknowledge that the current model of C₄ photosynthesis evolution is clearly the best available, C₃ -C₄ intermediates of hybrid origin, if existing, should not be used for further analysis of this model. However, experimental C₃  × C₄ hybrids potentially are excellent systems to analyse the genetic differences between C₃ and C₄ species and, also using segregating progeny, to study the relationship between individual photosynthetic traits and environmental factors.