Depression, recovery and facilitation of neuromuscular transmission during prolonged tetanic stimulation

The phrenic nerve of an unparalysed "cut" rat diaphragm preparation was stimulated with a rapid sequence of short tetanic trains. The amplitudes of both the first and the last (25th) endplate potentials produced by short tetanic trains progressively decreased with repeated application, but the latter diminished faster. Both relative depression and relative recovery (defined as fractional decrease and fractional recovery of endplate potential amplitudes that occur during and after each short train) became more pronounced with time. Relative facilitation (defined as fractional increase of endplate potential amplitudes observed at the beginning of each train) when initially present, persisted or increased slightly with duration of stimulation. Present results suggest that the "classic" depletion model for depression ought to be modified. They can be explained if it is assumed that during stimulation the capacity of the immediately available store to contain transmitter increases, and/or that newly formed transmitter preferentially replenishes the store of quanta immediately available for release. (An interesting consequence of the former model is that the immediately available store is replenished even when the small store behind is relatively more depleted.