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An attempt for fast separation of enantiomers in nano-liquid chromatography and capillary electrochromatography
Giovanni D'Orazio1, Rusudan Kakava2, Alessandro Volonterio3,4
1Institute of Chemical Methodologies, Italian National Research Council (CNR), Monterotondo, Italy.
Electrophoresis
|April 12, 2017
Abstract:
In the present study, an attempt was made to achieve separation of enantiomers within a minute in nano-LC and CEC. In order to achieve this goal several parameters were optimized from the viewpoint of the property of chiral analytes, concentration of the chiral selector in the packing material, capillary dimensions, and separation mode. The enantiomers of several of the applied chiral sulfoxides could be resolved with the analysis time <1 min. Some instrumental obstacles hindering further reduction of analysis time are also highlighted.