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Isolation Methods of Peripheral Blood Mononuclear Cells in Spanish Biobanks: An Overview
Irene Consuegra1, Clara Rodríguez-Aierbe2, Inés Santiuste3
11 Spanish HIV HGM BioBank, Hospital General Universitario Gregorio Marañón , Instituto de Investigación Sanitaria Gregorio Marañón, Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid, Spain .
Biopreservation and Biobanking
|April 12, 2017
Abstract:
The Spanish Hematic Derivatives Group, consisting of 26 biobanks, was established in 2011. We describe here the viability results of our publically available standard operating procedure to freeze and thaw peripheral blood mononuclear cells (PBMCs). Our protocol maximizes PBMC viability while avoiding where possible interbiobank and intrabiobank assay variability.