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Up-regulation of Let-7a Expression Induces Gastric Carcinoma Cell Apoptosis In Vitro
1Department of Surgery, College of Medicine and Health, Lishui University/Lishui People's Hospital, Lishui, Zhejiang 323000, China.
Abstract:
Objective To investigate effect of let-7a gene over-expression on apoptosis of gastric cancer cell lines SGC-7901.Methods The stable let-7a gene over-expressing SGC-7901 cells, SGC-7901/let-7a cells, were established using shRNA lentiviral vector methods. Real-time RT-PCR analysis was used to evaluate the expression level of let-7a mRNA. Cells apoptosis was assessed by flow cytometry.Results RT-PCR analysis revealed let-7a expression in SGC-7901/let-7a cells was significantly increased. Cellular apoptosis assay showed that over-expression of let-7a could increase apoptosis of SGC-7901 cells (P=0.002).Conclusion Up-regulating let-7a expression promoted apoptosis in SGC-7901 cells.
Insights
Over-expressing the let-7a gene significantly increased apoptosis in gastric cancer cells (SGC-7901). This finding suggests let-7a as a potential therapeutic target for gastric cancer treatment.
Area of Science:
- Molecular Biology
- Oncology
- Genetics
Background:
- Gastric cancer remains a leading cause of cancer-related mortality worldwide.
- Understanding the molecular mechanisms driving gastric cancer progression is crucial for developing effective therapies.
- The role of microRNAs, such as let-7a, in gastric cancer pathogenesis is an area of active investigation.
Purpose of the Study:
- To investigate the functional impact of let-7a gene over-expression on apoptosis in the SGC-7901 gastric cancer cell line.
- To determine if modulating let-7a levels can influence cancer cell death pathways.
Main Methods:
- Establishment of stable SGC-7901 cells with over-expressed let-7a (SGC-7901/let-7a) utilizing shRNA lentiviral vectors.
- Quantification of let-7a mRNA expression levels via real-time RT-PCR.
- Assessment of cellular apoptosis using flow cytometry.
Main Results:
- Real-time RT-PCR confirmed a significant up-regulation of let-7a expression in SGC-7901/let-7a cells compared to controls.
- Flow cytometry analysis demonstrated a statistically significant increase in apoptosis rates in SGC-7901 cells with let-7a over-expression (P=0.002).
Conclusions:
- Over-expression of the let-7a gene demonstrably promotes apoptosis in SGC-7901 gastric cancer cells.
- These findings highlight the potential of let-7a as a therapeutic agent or target for enhancing gastric cancer cell death.