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Effect of aminoglycoside concentration on reaction rates of aminoglycoside-modifying enzymes
G P Bongaerts1, J S Vliegenthart
1Department of Medical Microbiology, State University of Leiden, The Netherlands.
Antimicrobial Agents and Chemotherapy
|May 1, 1988
Abstract:
Reaction rates of several reference aminoglycoside-modifying enzymes were studied at various substrate concentrations. The resulting concentration-response curves showed wide variation in threshold concentration, in curve slope, in enzyme saturation, and in substrate inhibition. Together, the curves of a defined aminoglycoside panel yielded more specific information for each individual aminoglycoside-modifying enzyme tested than did conventional substrate profiles obtained at a single substrate concentration.