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Biopolymer based nanosystem for doxorubicin targeted delivery
Zsuzsanna Csikós1, Krisztina Kerekes1, Erika Fazekas1
1BBS Nanotechnology Ltd. Böszörményi 212., H-4032 Debrecen, Hungary.
American Journal of Cancer Research
|April 13, 2017
Summary
This study presents a novel, water-soluble drug delivery system using poly-γ-glutamic acid and chitosan nanoparticles for targeted doxorubicin delivery. This biopolymer-based system enhances anti-tumor efficacy and overall survival with reduced toxicity.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Drug Delivery Systems
Background:
- Development of effective drug delivery systems (DDS) is crucial for cancer therapy.
- Targeted drug delivery aims to increase therapeutic efficacy while minimizing systemic toxicity.
- Biocompatible and biodegradable polymers offer promising platforms for DDS construction.
Purpose of the Study:
- To create an actively and passively targeted, water-soluble DDS containing doxorubicin (DOX).
- To utilize poly-γ-glutamic acid (PGA) and chitosan (CH) for nanoparticle (NP) formation.
- To evaluate the stability, targeting, toxicity, and in vivo efficacy of the developed DDS.
Main Methods:
- Self-assembly of PGA and CH in aqueous solution to form nanoparticles.
- Functionalization of nanoparticles with folic acid (FA) for active targeting.
- Assessment of NP physical stability, in vitro cytotoxicity on folate receptor (FR)-positive cells, and drug release profile.
- In vivo anti-tumor efficacy studies and pharmacodynamic analysis.
Main Results:
- Stable nanoparticles (80-150 nm) with a slightly negative surface charge were formed.
- Targeted NPs demonstrated superior in vitro toxicity against FR-positive cells compared to free DOX or non-targeted NPs.
- The DDS exhibited stability during circulation and enhanced tumor growth inhibition in vivo.
- Reduced general toxicity and improved overall survival were observed with the biopolymer-based DDS.
Conclusions:
- The self-assembled, FA-targeted PGA-CH nanoparticles represent a stable and effective drug delivery system for doxorubicin.
- This biopolymer-based DDS shows significant potential for improving cancer treatment outcomes by enhancing tumor targeting and reducing side effects.