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Identification of a New Single-nucleotide Polymorphism within the Apolipoprotein A5 Gene, Which is Associated with
Samaneh Salehi1, Modjtaba Emadi-Baygi2, Majdaddin Rezaei3
1Department of Genetics, Faculty of Basic Sciences, Shahrekord University, Shahrekord, Iran.
Background:
Metabolic syndrome (MetS) is a common disorder which is a constellation of clinical features including abdominal obesity, increased level of serum triglycerides (TGs) and decrease of serum high-density lipoprotein-cholesterol (HDL-C), elevated blood pressure, and glucose intolerance. The apolipoprotein A5 (APOA5) is involved in lipid metabolism, influencing the level of plasma TG and HDL-C. In the present study, we aimed to investigate the associations between four INDEL variants of APOA5 gene and the MetS risk.
Materials And Methods:
In this case-control study, we genotyped 116 Iranian children and adolescents with/without MetS by using Sanger sequencing method for these INDELs. Then, we explored the association of INDELs with MetS risk and their clinical components by logistic regression and one-way analysis of variance analyses.
Results:
We identified a novel insertion polymorphism, c. *282-283 insAG/c. *282-283 insG variant, which appears among case and control groups. rs72525532 showed a significant difference for TG levels between various genotype groups. In addition, there were significant associations between newly identified single-nucleotide polymorphism (SNP) and rs72525532 with MetS risk.
Conclusions:
These results show that rs72525532 and the newly identified SNP may influence the susceptibility of the individuals to MetS.