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Improving the 'tool box' for robust industrial enzymes.

J A Littlechild1

  • 1Henry Wellcome Building for Biocatalysis, Biosciences, College of Life and Environmental Sciences, University of Exeter, Stocker Road, Exeter, EX4 4QD, UK. J.A.Littlechild@exeter.ac.uk.

Journal of Industrial Microbiology & Biotechnology
|April 13, 2017
PubMed
Summary

New microbial enzymes, identified through rapid sequencing and bioinformatics, offer sustainable

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Area of Science:

  • Industrial Biotechnology
  • Green Chemistry
  • Biocatalysis

Background:

  • Microbial genome and metagenome sequencing accelerates the discovery of novel biocatalysts.
  • Enzymes offer sustainable alternatives for chemical synthesis, waste breakdown, and carbon capture.
  • Industrial biotechnology increasingly relies on 'nature's catalysts' for eco-friendly processes.

Purpose of the Study:

  • To review recent advancements in identifying and characterizing high-priority industrial enzymes.
  • To highlight the role of hydrolase enzymes in industrial applications.
  • To present case studies from the Exeter group on enzyme discovery.

Main Methods:

  • Utilizing rapid microbial genome and metagenome sequencing.
  • Employing advanced bioinformatic approaches for enzyme identification.
Keywords:
Carbonic anhydrasesEpoxide hydrolasesEsterasesLactonasesThermophilic enzymes

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  • Developing new rapid enzyme activity screening methodologies.

    • Main Results:

    • Identification of robust biocatalysts for industrial biotechnology.
    • Demonstration of sustainable and environmentally friendly chemical synthesis routes.
    • Enzymes shown to be effective in carbon dioxide capture and waste stream valorization.

    Conclusions:

    • The continuous discovery of industrial enzymes is facilitated by advanced sequencing and bioinformatics.
    • Enzymes are key to developing a circular economy and green chemistry practices.
    • Specific hydrolase enzymes represent a significant resource for industrial applications.