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Updated: Jul 14, 2026

DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses
Published on: March 26, 2011
Analysis of DNA sequences in forty-year-old paraffin-embedded thin-tissue sections: a bridge between molecular
D Shibata1, W J Martin, N Arnheim
1Department of Pathology, University of Southern California Medical Center, Los Angeles 90033.
Abstract:
DNA sequences from human tissues paraffin embedded 40 yr ago were studied using the in vitro gene amplification technique known as the polymerase chain reaction. Although significant DNA degradation was observed, single copy genomic sequences and viral segments were readily detected from single 5- to 10-microns tissue sections. This demonstrates that the world-wide collection of archival paraffin-embedded tissues may be used to study the association of biological agents (viral, bacterial, or parasitic) or endogenous DNA lesions with disease over time and to carry out retrospective studies on material where the clinical outcome has already been established. This will be especially valuable in studying rare cancers and other rare diseases.
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