Characterization of oligonucleotide aptamers targeting the 5'-UTR from dengue virus

Elismar Jn Cnossen¹, Amanda G Silva¹, Karina Marangoni²

¹Molecular Biology Laboratory, Institute of Physics & Chemistry, & Institute of Biotechnology, Universidade Federal de Goias, Catalao, Brazil.

|April 14, 2017

Abstract

Aim:

The dengue virus is responsible for a high worldwide incidence of infections, aggravated by late diagnosis, and often confused with other tropical diseases. Results/methodology: Oligonucleotide aptamers binding to the 5'-UTR from dengue virus selected after eight rounds by systematic evolution of ligands by exponential enrichment technology were analyzed by dot-blot assay and in silico prediction of secondary structures, demonstrating the presence of stem-loops that may have the potential for interaction with the viral genome, which can lead to loss of their original conformation.

Conclusion:

This is the first description of RNA aptamers against functional RNA elements of the dengue virus genome with implications for disease control, which may have potential as tools in the future of antiviral therapies and for diagnostics.

Keywords:

SELEX dot-blot polymerase chain reaction reverse transcription untranslated region

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Characterization of oligonucleotide aptamers targeting the 5'-UTR from dengue virus

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Characterization of oligonucleotide aptamers targeting the 5'-UTR from dengue virus

Aim:

Conclusion:

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