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Updated: Aug 10, 2026

Measurement of Heme Synthesis Levels in Mammalian Cells
Published on: July 9, 2015
Haemoglobin site-specifically modified with ferulic acid to suppress the autoxidation
Donglai Qi1, Qian Li1, Pei Wang1
1a School of Environment and Chemical Engineering, Tianjin Polytechnic University , Tianjin , P.R. China.
Abstract:
Haemoglobin site-specifically modified with ferulic acid (FA-Hb), was synthesized. The effects on the characteristic absorption and the oxygen saturation curve of FA-Hb were investigated by UV-Vis spectrophotometry and oxygen analyzer. Furthermore, the autoxidation rates of Hb and FA-Hb were also discussed with or without sodium azide in the system. The results showed that there was no obvious transformation of the heme structure of FA-Hb. It was also demonstrated that the oxygen-carrying capacity of FA-Hb was similar to the natural Hb, but the autoxidation rate of FA-Hb was much lower than Hb in different systems.
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