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QTL mapping for fruit quality in Citrus using DArTseq markers
Maiara Curtolo1,2, Mariângela Cristofani-Yaly3, Rodrigo Gazaffi4
1Centro de Energia Nuclear na Agricultura - Universidade de São Paulo (USP), 13400-970, Piracicaba, SP, Brazil.
BMC Genomics
|April 14, 2017
Summary
This study developed a high-resolution genetic map for citrus using DArTseq markers, enabling quantitative trait locus (QTL) mapping for key fruit quality traits and identifying potential genes. This advances citrus breeding through biotechnological tools.
Area of Science:
- Plant genetics
- Genomics
- Biotechnology
Background:
- Citrus breeding faces limitations due to species biology.
- Biotechnological tools like DArTseq are crucial for advancing breeding.
- Next-generation sequencing aids in genetic map construction and QTL mapping.
Purpose of the Study:
- Construct an integrated genetic map for Murcott tangor and Pera sweet orange.
- Utilize DArTseq molecular markers for high-resolution mapping.
- Perform quantitative trait locus (QTL) mapping for twelve fruit quality traits.
Main Methods:
- Controlled crossing of Murcott tangor and Pera sweet orange.
- Genotyping of 278 F1 individuals using DArTseq markers.
- Integrated genetic map construction using OneMap software and comparison with the Citrus sinensis genome.
Main Results:
- An integrated map with 661 markers across 13 linkage groups (LGs) was created.
- Nineteen QTLs for twelve fruit quality traits were identified.
- Synteny analysis revealed putative genes associated with fruit quality traits.
Conclusions:
- An integrated linkage map using DArTseq markers was successfully established for citrus.
- The map is valuable for QTL mapping and identifying genes controlling fruit quality.
- This research contributes to assigning unmapped sequences within the Citrus sinensis reference genome.