Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A solid-phase reverse immunosorbent test for the detection of enterovirus IgM.

L O Magnius1, L H Saleh, T Vikerfors

  • 1Department of Virology, National Bacteriological Laboratory, Stockholm, Sweden.

Journal of Virological Methods
|May 1, 1988
PubMed
Summary

This study shows that the solid-phase reverse immunosorbent test (SPRIST) is a rapid and cost-effective method for detecting IgM antibodies against enteroviruses. The test demonstrated good sensitivity and cross-reactivity, aiding in the diagnosis of enterovirus infections.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Incidence of Hepatitis E Antibodies in Swedish Lung Transplant Recipients.

Transplantation proceedings·2015
Same author

[Molecular epidemiology of hepatitis C in centers of hemodialysis in St. Petersburg].

Zhurnal mikrobiologii, epidemiologii i immunobiologii·2015
Same author

Complete coding regions of the prototypes enterovirus B93 and C95: phylogenetic analyses of the P1 and P3 regions of EV-B and EV-C strains.

Journal of medical virology·2014
Same author

Immunomodulatory effects of secondary hyperparathyroidism on circulating CD4+ and CD8+ T-lymphocytes in chronic renal failure patients.

The Egyptian journal of immunology·2011
Same author

Molecular epidemiology of hepatitis E virus in humans, pigs and wild boars in Sweden.

Epidemiology and infection·2010
Same author

[Enteroviruses responsible for acute hemorrhagic conjunctivitis].

Medecine et maladies infectieuses·2009

Area of Science:

  • Virology
  • Immunology
  • Diagnostic Assays

Background:

  • Enterovirus infections pose a significant public health challenge, necessitating accurate and timely diagnostic methods.
  • Current diagnostic approaches for enteroviruses, such as virus isolation and complement fixation tests, can be time-consuming and may lack sensitivity in early disease stages.

Purpose of the Study:

  • To evaluate the utility of the solid-phase reverse immunosorbent test (SPRIST) for detecting IgM antibodies against enterovirus antigens.
  • To assess the sensitivity, specificity, and cross-reactivity of SPRIST in diagnosing enterovirus infections.

Main Methods:

  • The study utilized SPRIST to detect IgM antibodies against enterovirus antigens in 145 human sera.
  • Sera were collected from patients with enterovirus infections diagnosed by virus isolation or complement fixation (CF) antibody rise.

Related Experiment Videos

  • SPRIST performance was analyzed based on the timing of illness onset and compared with results from blood donors and rheumatoid factor-positive sera.

    • Main Results:

    • SPRIST demonstrated significant IgM antibody detection rates, with over 50% sensitivity during the second and third weeks post-illness onset.
    • The assay showed broad cross-reactivity, detecting antibodies against various enterovirus types (e.g., ECHO and Coxsackie viruses).
    • No reactivity was observed in sera with rheumatoid factor, and low false-positive rates were found in blood donor sera, indicating good specificity.

    Conclusions:

    • SPRIST is a rapid, convenient, and cost-effective mu-capture assay for enteroviruses.
    • The assay's cross-reactivity and sensitivity make it a valuable tool for diagnosing enterovirus infections, particularly during the acute phase.
    • SPRIST offers a promising alternative or adjunct to traditional diagnostic methods for enterovirus detection.