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Related Experiment Videos

Utilizing BD MAX™ Enteric Bacterial Panel to Detect Stool Pathogens from Rectal Swabs.

Barbara DeBurger1, Sarah Hanna1, Eleanor A Powell1

  • 1Cincinnati Children's Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229 USA.

BMC Clinical Pathology
|April 14, 2017
PubMed
Summary
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Rectal swabs can be used with the BD MAX™ Enteric Bacterial Panel (BDM-EBP) to accurately detect common enteric pathogens like Salmonella, Shigella, and Campylobacter. This study validates rectal swabs as acceptable specimens for BDM-EBP testing in pediatric patients.

Area of Science:

  • Clinical Microbiology
  • Molecular Diagnostics
  • Infectious Diseases

Background:

  • The BD MAX™ Enteric Bacterial Panel (BDM-EBP) is FDA-cleared for detecting Salmonella, Shigella, Campylobacter, and Shiga toxin genes from stool samples.
  • Rectal swabs are frequently used for pediatric enteric pathogen testing but are not FDA-cleared for use with the BDM-EBP.
  • This study aimed to assess the performance of the BDM-EBP when using rectal swabs as the specimen type.

Purpose of the Study:

  • To evaluate the efficacy of the BD MAX™ Enteric Bacterial Panel (BDM-EBP) in detecting enteric pathogens from rectal swabs.
  • To determine the accuracy and reliability of using rectal swabs as an alternative specimen for BDM-EBP testing in pediatric populations.

Main Methods:

  • 272 rectal swabs were tested using routine cultures, Shiga toxin testing, and the BDM-EBP.
Keywords:
BDM-Bacterial stool pathogensDiarrheaRectal swabs

Related Experiment Videos

  • Discrepant results were resolved using the Verigene® Enteric Pathogens Nucleic Acid Test (EP).
  • Performance was assessed using reference strains and patient isolates, including limit of detection studies.

    • Main Results:

    • The BDM-EBP demonstrated high Positive Percent Agreement (PPA) and Negative Percent Agreement (NPA) for Salmonella, Shigella, Campylobacter, and Shiga toxin-producing organisms when using rectal swabs.
    • After discrepant result resolution, PPAs ranged from 100% to 100%, and NPAs ranged from 95.3% to 100% for the tested pathogens.
    • Challenge samples were all detected, and limits of detection were established for each target organism in rectal swab matrix.

    Conclusions:

    • Rectal swabs are suitable and acceptable specimens for the detection of Salmonella, Shigella, Campylobacter, and Shiga toxin using the BD MAX™ Enteric Bacterial Panel (BDM-EBP).
    • The findings support the expanded clinical use of the BDM-EBP with rectal swabs, particularly for pediatric enteric pathogen testing.