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Genome editing: a robust technology for human stem cells.

Arun Pandian Chandrasekaran1, Minjung Song2, Suresh Ramakrishna3,4

  • 1Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul, Republic of Korea.

Cellular and Molecular Life Sciences : CMLS
|April 14, 2017
PubMed
Summary

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This summary is machine-generated.

Human stem cell genome editing utilizes advanced nucleases for precise DNA modification. These technologies accelerate the development of stem cells for crucial biological and therapeutic applications in disease models.

Area of Science:

  • Biotechnology
  • Genetics
  • Stem Cell Biology

Background:

  • Human pluripotent stem cells (hPSCs), including induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs), hold significant promise for biological research and therapeutic strategies.
  • Efficient genome alteration technologies are essential for harnessing the full potential of hPSCs in disease modeling and regenerative medicine.

Purpose of the Study:

  • To review recent technological advancements in genome editing using site-specific nucleases in human stem cells.
  • To highlight the role of these technologies in developing stem cell-based disease models.

Main Methods:

  • Discussion of synthetic nucleases for targeted genome modification.
  • Overview of advanced genome editing tools such as zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and CRISPR/Cas systems.
Keywords:
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated systemEmbryonic stem cellsInduced pluripotent stem cellsTranscription activator-like effector nucleasesZinc finger nucleases

Related Experiment Videos

  • Focus on applications in human stem cells.

    • Main Results:

    • Recent genome editing techniques have significantly enhanced the efficiency and speed of modifying stem cells.
    • Site-specific nucleases enable precise alterations within the DNA of human stem cells.
    • These advancements facilitate the creation of sophisticated stem cell models for studying human diseases.

    Conclusions:

    • Genome editing technologies, particularly site-specific nucleases, are pivotal for advancing stem cell research and applications.
    • The improved efficiency and precision of tools like CRISPR/Cas are transforming the development of hPSCs for therapeutic and research purposes.
    • Continued innovation in genome editing will further unlock the potential of stem cells in understanding and treating human diseases.