Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Chlorinated Flavonoids Modulate the Inflammatory Process in Human Blood.

Carina Proença1, Daniela Ribeiro1, Tânia Soares1

  • 1UCIBIO, REQUIMTE, Laboratory of Applied Chemistry, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, Rua de Jorge Viterbo Ferreira no. 228, 4050-313, Porto, Portugal.

Inflammation
|April 14, 2017
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

OT-55 reshapes tolerogenic BH3-mimetic-induced apoptosis toward immunogenic cell death in acute myeloid leukemia, potentiating PD-1/Tim-3 blockade.

Cell death & disease·2026
Same author

New Bicyclic Sesquiterpene and Labdane Diterpenes from the Culture Extract of the Sea Grass-Derived Fungus <i>Penicillium verruculosum</i> KUFA1509.

Marine drugs·2026
Same author

Adipose Tissue Plasticity and Adipogenesis: From Cellular Mechanisms to Therapeutic Targets in Obesity.

Current obesity reports·2026
Same author

The Structures Obtained from the Oxidation of 3-Amino-1<i>H</i>-indazole in Basic Conditions According to Hünig and Pozharskii.

The Journal of organic chemistry·2026
Same author

Exploring the Chemical Profile and Biological Activities of <i>Eryngium dichotomum</i>: UHPLC-MS/NMR Characterization, and In Vitro Antioxidant Activity Along with the Antitumor Effect of Falcarinol.

Molecules (Basel, Switzerland)·2026
Same author

Broad Screening of 2-Styrylchromones Identifies dual inhibitors of the COX-2 pathway.

Methods (San Diego, Calif.)·2026

Chlorinated flavonoids, metabolites of common flavonoids, show significant anti-inflammatory potential. These compounds effectively modulate inflammatory mediators and reactive species, suggesting therapeutic promise.

Area of Science:

  • Biochemistry
  • Pharmacology
  • Immunology

Background:

  • Flavonoids react with neutrophil-generated hypochlorous acid (HOCl) to form chlorinated products.
  • Some chlorinated flavonoids may retain or enhance inflammatory potential, necessitating further investigation.

Purpose of the Study:

  • To evaluate the anti-inflammatory potential of novel chlorinated flavonoids and parent compounds.
  • To assess their effects on cyclooxygenase (COX) activity, cytokine production (IL-6, IL-1β, TNF), chemokine (IL-8) release, and reactive species production.
  • To establish structure-activity relationships for these compounds.

Main Methods:

  • Integrated evaluation of chlorinated flavonoids and parent compounds.
  • In vitro study using human whole blood as a model.
Keywords:
anti-inflammatory effectchemokinescyclooxygenasecytokineshuman whole bloodreactive oxygen species

Related Experiment Videos

  • Assays for cyclooxygenase (COX)-1 and COX-2 activity.
  • Measurement of interleukin (IL)-6, IL-1β, tumor necrosis factor (TNF), and IL-8 production.
  • Assessment of reactive species production.

    • Main Results:

    • Luteolin was the most active compound among those tested.
    • Chlorinated flavonoids exhibited a notable pattern of activity in resolving inflammatory processes.
    • 6-chloro-3',4',5,7-tetrahydroxyflavone demonstrated significant ability to modulate reactive species and cytokine/chemokine production.

    Conclusions:

    • Chlorinated flavonoids possess considerable anti-inflammatory properties.
    • The metabolite 6-chloro-3',4',5,7-tetrahydroxyflavone warrants further scientific attention for its immunomodulatory effects.
    • The therapeutic potential of flavonoid metabolites, specifically chlorinated flavonoids, should be considered in drug development.