Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Pleiotropy01:33

Pleiotropy

Pleiotropy is the phenomenon in which a single gene impacts multiple, seemingly unrelated phenotypic traits. For example, defects in the SOX10 gene cause Waardenburg Syndrome Type 4, or WS4, which can cause defects in pigmentation, hearing impairments, and an absence of intestinal contractions necessary for elimination. This diversity of phenotypes results from the expression pattern of SOX10 in early embryonic and fetal development. SOX10 is found in neural crest cells that form melanocytes,...
Transcription01:10

Transcription

Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
Transcription01:17

Transcription

Transcription is the synthesis of RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in correctly synthesizing messenger RNA (mRNA). Transcriptional regulation is responsible for the differentiation of different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
General Transcription Factors01:30

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Self-Sacrificial Sustained-Release Effects Synergistically Modulate Dual-Electrode Interfacial Chemistry in a Biphasic Electrolyte for Electrolytic Zn-MnO<sub>2</sub> Batteries.

ACS applied materials & interfaces·2026
Same author

Gelatin-Syringaldehyde-Zinc Hydrogel: A Triple-Action Bioactive Dressing for Infected Wound Regeneration.

ACS applied bio materials·2026
Same author

Mung Bean Protein-Derived Bioink with Synergistic Antioxidant and Antibacterial Properties for Cartilage Regeneration.

ACS applied materials & interfaces·2026
Same author

Association between immune-inflammatory biomarkers (NLR, PLR, SII, SIRI) and obesity in adults: a systematic review and meta-analysis.

International journal of obesity (2005)·2026
Same author

A Photothermally Active CuS-Nanocomposite Hydrogel for Postsurgical Melanoma Management and Tissue Regeneration.

ACS applied bio materials·2026
Same author

Combination of Triglyceride Glucose Index and Obesity-Related Parameters for Predicting Nonalcoholic Fatty Liver Disease in Overweight/Obese Men.

Current medicinal chemistry·2026
Same journal

Restraint of melanoma progression by cells in the local skin environment.

eLife·2026
Same journal

Brawn before bite in endemic Asian eutherian mammals after the end-Cretaceous extinction.

eLife·2026
Same journal

Experimental evolution to thermal stress indicates climate resilience in a cosmopolitan arthropod.

eLife·2026
Same journal

Correlates of protection against African swine fever virus identified by a systems immunology approach.

eLife·2026
Same journal

Retrosplenial cortex enables context-dependent goal-directed sensorimotor transformation.

eLife·2026
Same journal

Direct contact between iPSC-derived macrophages and hepatocytes drives reciprocal acquisition of Kupffer cell identity and hepatocyte maturation.

eLife·2026
See all related articles

Related Experiment Video

Updated: Jun 23, 2026

Quantifying the Activity of cis-Regulatory Elements in the Mouse Retina by Explant Electroporation
07:38

Quantifying the Activity of cis-Regulatory Elements in the Mouse Retina by Explant Electroporation

Published on: June 28, 2011

Evolutionary changes in transcription factor coding sequence quantitatively alter sensory organ development and

Simon Weinberger1,2,3, Matthew P Topping4,5, Jiekun Yan1,2

  • 1VIB Center for the Biology of Disease, VIB, Leuven, Belgium.

Elife
|April 14, 2017
PubMed
Summary
This summary is machine-generated.

Coding sequences of Atonal homologues (ATHs) can rescue sensory organ development in flies. Protein stability, not mRNA levels, drives functional differences in proneural activity.

Keywords:
D. melanogasterEvoDevoatonal homologuesdevelopmental biologyevolutionary biologygenomicsneurogenesisstem cells

More Related Videos

An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides
09:58

An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides

Published on: November 29, 2016

An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations
11:36

An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations

Published on: April 21, 2023

Related Experiment Videos

Last Updated: Jun 23, 2026

Quantifying the Activity of cis-Regulatory Elements in the Mouse Retina by Explant Electroporation
07:38

Quantifying the Activity of cis-Regulatory Elements in the Mouse Retina by Explant Electroporation

Published on: June 28, 2011

An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides
09:58

An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides

Published on: November 29, 2016

An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations
11:36

An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations

Published on: April 21, 2023

Area of Science:

  • Evolutionary developmental biology
  • Molecular evolution
  • Genetics

Background:

  • Morphological evolution is driven by cis-regulatory changes in developmental genes.
  • The contribution of coding sequence evolution to animal evolution is not fully understood.

Purpose of the Study:

  • To investigate the role of coding sequence evolution in the Atonal gene family.
  • To determine if Atonal homologues (ATHs) can functionally replace Drosophila atonal.
  • To explore the impact of coding sequence variations on sensory organ development.

Main Methods:

  • Endogenous replacement of Drosophila atonal coding sequence with various ATHs and other proneural genes.
  • Assessing the rescue of sensory organ fate in atonal mutants.
  • Quantifying proneural activity and correlating it with protein stability and signaling responses.

Main Results:

  • ATHs and ancestral-like coding sequences rescued sensory organ development, unlike non-ATHs.
  • Different ATHs exhibited varying levels of proneural activity, forming a potency gradient.
  • ATH protein stability, particularly in response to Notch signaling, correlated with proneural potency, independent of mRNA levels or codon usage.

Conclusions:

  • Coding sequence evolution plays a significant role in quantitative variation in sensory development.
  • ATH proteins possess distinct and ancient functions influencing sensory organ development.
  • Protein stability is a key factor in the functional divergence of proneural genes.