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In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells
05:57

In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells

Published on: May 16, 2011

Streptococcal Adhesin P (SadP) contributes to Streptococcus suis adhesion to the human intestinal epithelium

Maria Laura Ferrando1,2, Niels Willemse1,2, Edoardo Zaccaria3

  • 1Department of Medical Microbiology, Center for Infection and Immunity, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.

Plos One
|April 14, 2017
PubMed
Abstract

Insights

Streptococcus suis adhesin protein (SadP) aids bacterial entry into the gastrointestinal tract. Variants of SadP influence adhesion to host cells, impacting the zoonotic potential of this pathogen.

Area of Science:

  • Microbiology
  • Pathogen-Host Interactions
  • Zoonotic Diseases

Background:

  • Streptococcus suis is a zoonotic pathogen causing meningitis and septicemia.
  • The gastrointestinal tract (GIT) is a key entry site for zoonotic S. suis infections.
  • The role of Streptococcal adhesin Protein (SadP) in GIT-level host-pathogen interactions requires further investigation.

Purpose of the Study:

  • To investigate the contribution of SadP to host-pathogen interactions at the GIT level.
  • To analyze SadP variants and their role in S. suis adhesion to intestinal epithelial cells (IEC).

Main Methods:

  • Quantitative Real Time PCR (qRT-PCR) to measure sadP expression with IEC.
  • Phylogenetic analysis of complete DNA sequences to identify SadP variants.
  • In vitro testing of SadP knockout and complementation mutants with IEC.

Main Results:

  • SadP expression significantly increased in the presence of IEC.
  • Five SadP sequence variants were identified, correlating with genotype.
  • SadP1 variant facilitated binding and translocation across human IEC, with inhibition by anti-Gb3/CD77 antibodies.

Conclusions:

  • SadP plays a crucial role in host-pathogen interactions within the GIT.
  • SadP variants exhibit differential affinities for the Gb3/CD77 receptor, affecting adhesion to host IEC.
  • These variations in adhesion capacity contribute to the zoonotic potential of S. suis.

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