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Related Experiment Videos

Aminoglycoside resistance patterns in Turkey.

H E Akalin1, M Torun, R Alacam

  • 1Department of Medicine, Hacettepe University, School of Medicine, Ankara, Turkey.

Scandinavian Journal of Infectious Diseases
|January 1, 1988
PubMed
Summary
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Aminoglycoside resistance in gram-negative bacteria varies regionally, often due to modifying enzymes. Understanding these resistance patterns is crucial for effective antibiotic use and combating infections.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Pharmacology

Background:

  • Gram-negative bacterial resistance to aminoglycoside antibiotics presents a significant global health challenge.
  • Regional variations in resistance prevalence necessitate localized surveillance and understanding of underlying mechanisms.
  • Aminoglycoside-modifying enzymes are the primary drivers of resistance in gram-negative bacilli.

Purpose of the Study:

  • To evaluate resistance mechanisms in aminoglycoside-resistant gram-negative bacteria.
  • To compare resistance patterns between community-acquired and hospital-acquired infections.
  • To identify prevalent aminoglycoside-modifying enzymes contributing to resistance.

Main Methods:

  • Susceptibility testing of 300 aminoglycoside-resistant gram-negative bacterial isolates to selected aminoglycosides.

Related Experiment Videos

  • Determination of aminoglycoside resistance mechanisms, specifically identifying modifying enzyme types.
  • Comparative analysis of resistance profiles from community and hospital-acquired infection isolates.

    • Main Results:

    • The 2''-adenyltransferase [ANT(2'')] enzyme was the most common resistance mechanism identified (45.4% community, 44.4% hospital).
    • The 3-acetyltransferase [AAC(3)-II] was the second most prevalent enzyme (20.8% community, 23.3% hospital) and most common in Pseudomonas aeruginosa.
    • Overall prevalence followed ANT(2''), then AAC(3)-II, and AAC(3)-I, indicating significant enzyme-mediated resistance.

    Conclusions:

    • Local antibiotic prescribing patterns significantly influence the prevalence of specific aminoglycoside resistance mechanisms.
    • ANT(2'') and AAC(3)-II are key enzymes contributing to aminoglycoside resistance in the studied gram-negative bacteria.
    • Findings underscore the need for regional monitoring of antibiotic resistance to guide therapeutic strategies.