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Updated: Jun 16, 2026
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Skin Punch Biopsy Explant Culture for Derivation of Primary Human Fibroblasts
Published on: July 7, 2013
Transfection of Primary Human Skin Fibroblasts for Peroxisomal Studies
Janet Koster1, Hans R Waterham2
1Laboratory Genetic Metabolic Diseases, Department of Clinical Chemistry, Academic Medical Center, University of Amsterdam, Meibergdreef 9, 1105 AZ, Amsterdam, The Netherlands.
Methods in Molecular Biology (Clifton, N.J.)
|April 15, 2017
Abstract:
Functional studies with primary human skin fibroblasts from patients with a peroxisomal disorder often require efficient transfection with plasmids to correct the genetic defect or to express heterologous reporter proteins. Here, we describe a protocol we commonly use for efficient nonviral transfection of primary human skin fibroblasts.
