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Detection of Ubiquitinated Peroxisomal Proteins in Yeast
Natasha Danda1, Chris Williams2
1Molecular Cell Biology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, 9700 CC, Groningen, The Netherlands.
Methods in Molecular Biology (Clifton, N.J.)
|April 15, 2017
Abstract:
Ubiquitination is involved in different aspects of peroxisome formation, maintenance, and degradation. Consequently, simple methods for detecting ubiquitinated peroxisomal proteins are extremely useful in peroxisomal research. Here, we describe an immunoprecipitation-based technique that can be used to assess peroxisomal protein ubiquitination in yeast.

