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Updated: Apr 22, 2026

High-resolution Imaging and Analysis of Individual Astral Microtubule Dynamics in Budding Yeast
Published on: April 20, 2017
[Modern methods in analysis of microtubules dynamic in vivo]
Marta Ewelina Wawro1, Katarzyna Wieczorek1,2, Katarzyna Sobierajska1
1Medical University of Lodz; Department of Molecular Cell Mechanisms, 6/8 Mazowiecka St., 92-215 Lodz, Poland.
Abstract:
Microtubules are involved in any vital cellular activities, including the maintenance of cell shape, division, migration and intracellular transport. Microtubule dynamics is regulated by the balance between their polymerization and depolymerization. Microtubule stability is dependent on their alpha and beta subunits composition, tubulin post-translational modifications and interaction of microtubules with microtubule-associated proteins (MAPs). Disruption of these processes can lead to a number of pathological conditions such as cancer, cardiovascular disease, or the fibrosis development. This review summarizes the current knowledge of the modern methods of microtubule polymerization analysis. This allows a better understanding of the structure and mechanisms played by microtubules in their physiological functions and the development of pathological conditions resulting from their disorder.
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