Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Glycogen phosphorylase inhibition improves beta cell function.

Lilla Nagy1,2, Judit Márton1, András Vida1,3

  • 1Department of Medical Chemistry, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.

British Journal of Pharmacology
|April 15, 2017
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

TOP1MT rs2293925 is an enhancer-active regulatory SNP that shapes mitochondrial R-loop dynamics.

The FEBS journal·2026
Same author

The Pathophysiological Interrelationship Between Metabolic Dysfunction-Associated Steatotic Liver Disease and Cardiovascular Disease.

Antioxidants (Basel, Switzerland)·2026
Same author

Myofilament-level effects of aficamten increase diastolic chamber volumes and maintain cardiac output through preserved length-dependent force generation in healthy rat and canine myocardium.

Basic research in cardiology·2026
Same author

Comparison of some economic traits by genetic cluster of Aberdeen Angus cattle.

Archives animal breeding·2026
Same author

Tissue-specific autoantibody signatures reveal immune alterations undetected by routine serology in long COVID.

GeroScience·2026
Same author

ZCWPW1 organizes telomeric architecture to drive meiotic chromosome movements.

bioRxiv : the preprint server for biology·2026

Glycogen phosphorylase inhibitors (GPi-s) enhance glucose-stimulated insulin secretion and preserve beta cell function. These findings suggest GPi-s can be repurposed to treat diabetes by improving beta cell function.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Endocrinology

Background:

  • Glycogen phosphorylase (GP) is crucial for glycogen breakdown.
  • GP inhibitors (GPi-s) increase liver glycogen storage.
  • Glycogen metabolism impacts beta cell function and insulin secretion.

Purpose of the Study:

  • To investigate the effect of GPi-s on beta cell function.
  • To determine if modulating glycogen metabolism influences beta cell activity.

Main Methods:

  • Experiments conducted on MIN6 insulinoma cells and a mouse model of diabetes.
  • Assessed changes in glycogen content, protein phosphorylation, gene expression, and insulin secretion.
  • Utilized in silico screening to examine insulin receptor interactions.

Related Experiment Videos

Main Results:

  • GPi treatment increased glycogen content and surface area in MIN6 cells.
  • GPi-s enhanced insulin receptor signaling (InsRβ, Akt, p70S6K phosphorylation) and PDX1/insulin expression.
  • GPi-s improved both non-stimulated and glucose-stimulated insulin secretion in vitro and in vivo, increasing islet size in mice.

Conclusions:

  • GPi-s directly impact beta cells, suggesting a role in preserving or improving their function.
  • GPi-s show potential for repurposing as therapeutic agents for diabetes.
  • Drug repurposing of GPi-s offers a novel strategy for managing beta cell dysfunction in diabetes.