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Related Experiment Videos

EpCAM controls morphogenetic programs during zebrafish pronephros development.

Sebastian Kuechlin1, Maximilian Schoels1, Krasimir Slanchev1

  • 1Renal Division, Department of Medicine, University Freiburg Medical Center, Hugstetter Str. 55, 79106 Freiburg, Germany.

Biochemical and Biophysical Research Communications
|April 16, 2017
PubMed
Summary

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This summary is machine-generated.

Epithelial cell adhesion molecule (EpCAM) is crucial for early kidney development. Zebrafish studies reveal EpCAM deficiency impacts gene expression, affecting kidney morphogenesis and cell differentiation.

Area of Science:

  • Developmental Biology
  • Renal Physiology
  • Molecular Biology

Background:

  • Epithelial cell adhesion molecule (EpCAM) is a transmembrane glycoprotein.
  • EpCAM expression is dynamic in human and murine renal epithelia during development.
  • EpCAM levels increase during renal regeneration and in carcinomas, but its role in early kidney development is unclear.

Purpose of the Study:

  • To investigate the role of EpCAM in early kidney development using zebrafish as a model.
  • To analyze global gene expression changes in EpCAM-deficient zebrafish pronephros.

Main Methods:

  • Utilized zebrafish pronephros, which shares segmentation patterns with mammalian nephrons.
  • Developed a method for global gene expression analysis in specific pronephric segments.
Keywords:
Distal tubuleEpcamLaser-capture microdissectionPronephros developmentRNA-seqZebrafish

Related Experiment Videos

  • Compared gene expression between wild-type and EpCAM-deficient zebrafish embryos.

    • Main Results:

    • Identified 379 differentially expressed genes in EpCAM-mutant zebrafish.
    • Gene ontology analysis revealed EpCAM's control over ureteric bud development.
    • EpCAM influences branching morphogenesis, cell differentiation, and cilium morphogenesis.

    Conclusions:

    • EpCAM plays a significant role in regulating gene expression during early kidney development.
    • EpCAM is essential for normal pronephric development, including ureteric bud formation and epithelial morphogenesis.
    • EpCAM-deficient zebrafish exhibit defects in cell differentiation and cilium formation, highlighting its broad developmental functions.