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Related Experiment Videos

Global exosome transcriptome profiling reveals biomarkers for multiple sclerosis.

Igor Selmaj1, Maria Cichalewska1, Magdalena Namiecinska1

  • 1Laboratory of Neuroimmunology, Department of Neurology, University of Lodz, Lodz, Poland.

Annals of Neurology
|April 16, 2017
PubMed
Summary

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Circulating exosomal microRNAs (miRNAs) are altered in relapsing-remitting multiple sclerosis (RRMS). Decreased levels of specific exosomal miRNAs during RRMS relapse may serve as a biomarker for disease activity.

Area of Science:

  • Neuroimmunology
  • Molecular Biology
  • Biomarker Discovery

Background:

  • Exosomes play a role in immune regulation.
  • Understanding the exosome transcriptome in multiple sclerosis is crucial for immune regulation insights.

Purpose of the Study:

  • To investigate the circulating exosome transcriptome in relapsing-remitting multiple sclerosis (RRMS) patients.
  • To identify differentially expressed microRNAs (miRNAs) in exosomes from RRMS patients compared to healthy controls.

Main Methods:

  • Serum exosomes were isolated from RRMS patients and healthy controls.
  • Next-generation sequencing (NGS) was employed to profile the exosomal RNA.
  • Digital quantitative polymerase chain reaction (dqPCR) was used for validation in an independent cohort.

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Main Results:

  • Four specific microRNAs (miRNAs) were found to be differentially expressed in RRMS patients.
  • Serum exosomal expression of these miRNAs was significantly decreased during RRMS relapse.
  • Impaired in vitro secretion of these miRNAs by peripheral blood mononuclear cells was observed in RRMS patients.

Conclusions:

  • Circulating exosomes exhibit a distinct RNA profile in RRMS.
  • Decreased exosomal miRNAs suggest disturbed cell-to-cell communication in RRMS.
  • Exosomal miRNAs may serve as potential biomarkers for distinguishing multiple sclerosis relapse.