Related Experiment Videos
Heterogeneity in an isolated membrane protein. Has the 'authentic cytochrome oxidase' been identified?
C R Hartzell1, H Beinert, G T Babcock
1Department of Research, Alfred I. DuPont Institute, Wilmington, DE 19899.
FEBS Letters
|August 15, 1988
Abstract:
The criteria of homogeneity or native state of a protein are prone to become ambiguous when applied to membrane proteins, such as cytochrome-c oxidase, which are purified by extraction with detergents. Properties of the purified material depend on the detergent used and on details of the purification protocol followed with any single batch of a preparation. We present arguments to show that the evidence presently available in published form does not justify the designation [(1987) J. Biol. Chem. 262, 3160-3164] of one type of preparation as being closer to the native state than others.