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Related Experiment Videos

Enzyme Adsorption on Nanoparticle Surface Probed by Highly Sensitive Second Harmonic Light Scattering.

Anindita Das1, Abhijit Chakrabarti2, Puspendu K Das1

  • 1Indian Institute of Science, Bangalore, India.

Methods in Enzymology
|April 17, 2017
PubMed
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Second harmonic light scattering reveals protein-nanoparticle interactions. This method quantizes binding thermodynamics, crucial for developing nanoparticle drug delivery systems.

Area of Science:

  • Surface science
  • Biophysics
  • Nanotechnology

Background:

  • Understanding molecular interactions on nanoparticle surfaces is key for biomedical applications.
  • Second harmonic light scattering (SHLS) offers insights into these surface interactions.
  • Protein adsorption thermodynamics on nanoparticles is critical for formulation stability and efficacy.

Purpose of the Study:

  • To extend the second harmonic light scattering technique for probing protein adsorption thermodynamics on nanoparticle surfaces.
  • To assess the applicability of SHLS for characterizing protein-nanoparticle interactions in biomedical contexts.
  • To determine thermodynamic parameters like free energy change, association constant, and binding stoichiometry.

Main Methods:

  • Utilized the second harmonic light scattering technique.
Keywords:
AdsorbateAdsorptionBinding constantEnzyme–nanoparticle conjugateFree energyGold nanoparticleLangmuir isotherm

Related Experiment Videos

  • Applied a modified Langmuir adsorption model to analyze experimental data.
  • Investigated the adsorption of alcohol dehydrogenase and insulin on gold nanoparticles.

    • Main Results:

    • Successfully extracted thermodynamic parameters, including free energy change, association constant, and binding stoichiometry.
    • Determined a free energy change of approximately -55 kJ/mol, indicating weak protein-nanoparticle interactions.
    • Demonstrated the reversibility of the protein binding process due to low free energy changes.

    Conclusions:

    • The second harmonic light scattering technique is broadly applicable for studying protein adsorption thermodynamics on nanoparticles.
    • The findings provide insights into the weak, reversible interactions between proteins and nanoparticle surfaces.
    • This method enables control over protein payload for future protein- or peptide-based therapeutic applications.