Armoring Enzymes by Metal-Organic Frameworks by the Coprecipitation Method

Enzymatic processes have great potential for applications in industrial biocatalysis, biosensing, and biomedical engineering. However, poor stability and difficulty in the reuse of enzymes still create challenges for their use in catalysis and other applications. In recent years, enzyme-incorporated metal-organic frameworks (enzyme@MOF) have emerged as a new type of armored enzymes and are thought to be an appealing solution to the above challenges. To date, the preparation of enzyme-MOF composites can be divided into three categories, including physical adsorption, covalent conjugation, and coprecipitation. The coprecipitation method integrates the synthesis of MOFs and encapsulation of enzymes into one step, allowing common MOFs with regular pore sizes to be used as carriers for enzyme binding. In this chapter, we present a protocol for the synthesis of enzyme@MOF composites using the coprecipitation approach with particular details.