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Isolation and characterization of the porcine nuclear factor I (NFI) gene
M Meisterernst1, L Rogge, C Donath
1Institut für Biochemie der Universität München, FRG.
FEBS Letters
|August 15, 1988
Abstract:
This study describes the isolation of a major portion of the gene for nuclear factor I (NFI) including its 5'-flanking region with transcriptional start sites. We screened a porcine liver, genomic DNA library in phage EMBL3A with synthetic oligonucleotides derived from tryptic and cyanogen-bromide peptide sequences obtained from purified NFI protein. The NFI gene is present as a single copy in porcine DNA.