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Updated: Aug 17, 2026

Methylated DNA Immunoprecipitation
Published on: January 2, 2009
A quantitative method for detecting DNA methylation over targeted genomic regions using isotope dilution liquid
Xiaoxia Ye1, Lijian Zhang2, Bin Chen2
1Laboratory for Genetics, Nutrition and Health, Guangdong Medical University, Zhanjiang, Guangdong 524023, PR China.
Abstract:
Aberrant DNA methylation is associated with various diseases. Quantitative analysis of regional DNA methylation levels of some specific genes would aid in diseases diagnosis and risk stratification. In this study, we developed a robust method for detecting DNA methylation level over targeted genomic regions using nucleobases quantification in bisulfite amplicons by isotope dilution liquid chromatography tandem mass spectrometry coupled with a simple equation. This method had wide detection range (from 0% to 100% methylation) and high accuracy while more time-saving compared to clonal bisulfite sequencing method. The application for clinical tissue samples showed good applicability and cost effectiveness. This analytical method is suitable for quantifying average DNA methylation level over targeted genomic regions and expected to be a useful tool for detecting DNA methylation biomarkers.

