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Related Experiment Videos

Allelic and ectopic recombination between Ty elements in yeast.

M Kupiec1, T D Petes

  • 1Department of Molecular Genetics and Cell Biology, University of Chicago, Illinois 60637.

Genetics
|July 1, 1988
PubMed
Summary

Ty element recombination occurs at normal frequencies in meiosis and mitosis. Ectopic recombination is more varied in mitosis than meiosis, with homology length sufficient for various recombination events.

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Genetics·2001

Area of Science:

  • Molecular Biology
  • Genetics
  • Yeast Biology

Background:

  • Transposable elements, such as Ty elements in yeast, are mobile genetic sequences that can undergo recombination.
  • Understanding recombination mechanisms is crucial for comprehending genome stability and evolution.

Purpose of the Study:

  • To investigate the frequencies and types of allelic and nonallelic (ectopic) recombination involving marked Ty elements.
  • To compare recombination patterns during mitosis and meiosis.

Main Methods:

  • Analysis of recombination events in isogenic yeast strains carrying marked Ty elements.
  • Distinguishing between allelic and ectopic recombination events.
  • Observing recombination during both mitotic and meiotic cell divisions.

Main Results:

  • Allelic recombination occurred at normal frequencies in both meiosis and mitosis.
  • Ty elements participated in a wide array of ectopic recombination types, with greater variety observed in mitosis.
  • Allelic and ectopic recombination happened at similar frequencies in mitosis, but allelic recombination was predominant in meiosis.
  • Evidence for concerted recombination events was observed in ectopic mitotic recombination.
  • A homology length of 330 bp (delta element) was sufficient for certain meiotic and mitotic recombination events.

Conclusions:

  • Ty element recombination, both allelic and ectopic, occurs efficiently in yeast.
  • The cellular division stage (mitosis vs. meiosis) influences the type and frequency of Ty element recombination.
  • Even short homology regions, like those in delta elements, can facilitate recombination.

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