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In vitro cytotoxicity of decabrominated diphenyl ether (PBDE-209) to human red blood cells (hRBCs)
Zhenxing Chi1, Songwen Tan2, Weiguo Li2
1School of Marine Science and Technology, Harbin Institute of Technology, Weihai, 2# Wenhua West Road, Weihai, 264209, PR China; State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, 73# Huanghe Road, Harbin, 150090, PR China; Guangzhou Key Laboratory of Environmental Exposure and Health, School of Environment, Jinan University, Guangzhou, 510632, PR China.
Abstract:
This work presents the effect of decabrominated diphenyl ether (PBDE-209) on the anti-oxidative defense capacity, and ATPase activity (structure and function) of human red blood cells (hRBCs). The results show that the PBDE-209 influences the activity and content of typical biomolecules (SOD, CAT, GSH-Px, GSH and MDA) in hRBCs, causing a decline in the function of the antioxidant defense system. The PBDE-209 with a concentration of 10 μmol/L resulted in the cytoplasmic projections and structure deformation of the hRBCs. When its concentration exceeds 25 μmol/L, the relative ATPase activity was decreased to 20% of the initial activity. Since the discovered effects of PBDE-209 on hRBCs are in cell level, this study may offer some information to advise the related in vivo cytotoxicity works.