Related Experiment Videos
Generation of a Nrf2 homozygous knockout human embryonic stem cell line using CRISPR/Cas9
So-Jung Kim1, Omer Habib2, Jin-Soo Kim2
1Division of Intractable Diseases, Center for Biomedical Sciences, Korea National Institute of Health, Cheongju 28159, Republic of Korea; Department of Program in Cell Biology and Genetics, College of Medicine, Chungbuk National University, Cheongju 361-763, Republic of Korea.
Abstract:
Nuclear factor erythroid 2-related factor 2 (NFE2L2 or Nrf2) is a well-known transcription factor that regulates the expression of a large number of anti-oxidant genes in mammalian cells (J.H. Kim et al., 2014). Here, we generated a homozygous Nrf2 knockout human embryonic stem cell (hESC) line, H9Nrf2KO-A13, using the CRISPR/Cas9 genome editing method. The Nrf2 homozygous knockout H9 cell line maintains pluripotency, differentiation potential into three germ layers, and a normal karyotype.