Related Experiment Videos
A homozygous Keap1-knockout human embryonic stem cell line generated using CRISPR/Cas9 mediates gene targeting
So-Jung Kim1, Omer Habib2, Jin-Soo Kim2
1Division of Intractable Diseases, Center for Biomedical Sciences, Korea National Institute of Health, Cheongju 28159, Republic of Korea; Department of Program in Cell Biology and Genetics, College of Medicine, Chungbuk National University, Cheongju 361-763, Republic of Korea.
Abstract:
Kelch-like ECH-associated protein 1 (keap1) is a cysteine-rich protein that interacts with transcription factor Nrf2 in a redox-sensitive manner, leading to the degradation of Nrf2 (Kim et al., 2014a). Disruption of Keap1 results in the induction of Nrf2-related signaling pathways involving the expression of a set of anti-oxidant and anti-inflammatory genes. We generated biallelic mutants of the Keap1 gene using a CRISPR-Cas9 genome editing method in the H9 human embryonic stem cell (hESC). The Keap1 homozygous-knockout H9 cell line retained normal morphology, gene expression, and in vivo differentiation potential.