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Related Experiment Videos

Cell Isolation and Recovery Using Hollow Glass Microspheres Coated with Nanolayered Films for Applications in

Ziye Dong1, Caroline C Ahrens1, Dan Yu2

  • 1Department of Chemical Engineering, Texas Tech University , Lubbock, Texas 79409, United States.

ACS Applied Materials & Interfaces
|April 18, 2017
PubMed
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This study introduces a novel, power-free method for cell isolation using self-floating microspheres. This technique enables efficient cell capture and release, ideal for point-of-care diagnostics in remote settings.

Area of Science:

  • Biotechnology
  • Materials Science
  • Cell Biology

Background:

  • Existing cell isolation methods like fluorescence-activated cell sorting (FACS) and microfluidics require specialized equipment and power, limiting their use in remote or resource-limited settings.
  • Point-of-care diagnostics in remote areas face challenges due to the lack of laboratory infrastructure and consistent power sources.

Purpose of the Study:

  • To develop a simple, power-free, and disposable cell isolation and recovery technology suitable for point-of-care applications in resource-limited environments.
  • To demonstrate the efficacy of self-floating microspheres coated with functionalized nanolayers for targeted cell capture and release.

Main Methods:

  • Development of self-floating hollow glass microspheres coated with an enzymatically degradable nanolayered film and conjugated with antibodies.
Keywords:
buoyancycell isolationcell recoveryglass microsphereslayer-by-layernanolayered filmresource-limited settings

Related Experiment Videos

  • Grafting an antifouling polymer brush layer (poly(ethylene glycol)) onto the nanolayered film to enhance purity.
  • Demonstration of cell isolation using EpCAM-expressing PC-3 cancer cells in blood as a model system.

    • Main Results:

    • Successful isolation and recovery of targeted cancer cells from a mixture using antibody-conjugated, self-floating microspheres.
    • Demonstrated minimal impact on cell viability and proliferative potential post-isolation.
    • Achieved high purity of isolated cells through the antifouling polymer brush layer.

    Conclusions:

    • The developed microsphere-based technology offers a viable solution for cell isolation and recovery without specialized equipment or power.
    • This approach has the potential to overcome practical limitations in resource-limited settings, enabling downstream analyses of viable targeted cells.
    • The method is rapid, completing the process in under 1 hour, making it suitable for time-sensitive point-of-care applications.