In vitro cytotoxicity evaluation of nano-carbon particles with different sp²/sp³ ratios

Graphitization occurs during the long-term service of a diamond-like carbon (DLC) modified artificial joint. Then, DLC wear debris, which are carbon particles with different sp²/sp³ ratios and sizes ranging from the nano- to micro-meter scale produced. In this paper, to promote the application of DLC coating for artificial joint modification, the cytotoxicity of DLC debris (nano-carbon particles, NCs) with different sp²/sp³ ratios was studied. The microstructure and physical characteristics of NCs with different sp²/sp³ ratios were investigated by Raman spectroscopy, X-ray photoelectron spectroscopy (XPS), Transmission Electron Microscope (TEM) and Dynamic Light Scattering (DLS). Meanwhile, osteoblasts and macrophages were applied to characterize the cytotoxicity of the NCs. In vitro cytotoxicity assay results indicated that cells incubated with NCs of different sp²/sp³ ratios had greater osteogenic capacity, and these particles caused a weaker immune response in comparison with CoCrMo particles. Taken together, the results indicated that NCs with different sp²/sp³ ratios presented a good cytocompatibility than CoCrMo particles. But no significant differences were observed among NCs with different sp²/sp³ ratios. The better cytocompatibility of NCs is mainly attributable to their surface charge.