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Related Experiment Videos

Overcoming "speed limits" in high throughput chromatographic analysis.

Kerstin Zawatzky1, Chandan L Barhate1, Erik L Regalado1

  • 1Merck & Co., Inc., Process Research & Development, Rahway, NJ 07065, USA.

Journal of Chromatography. A
|April 19, 2017
PubMed
Summary

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High-speed autosamplers and ultrafast chromatography significantly boost high throughput analysis. Multiple Injection in a Single Experimental Run (MISER) HPLC-MS can analyze a 96-well plate in just 17 minutes.

Area of Science:

  • Analytical Chemistry
  • Biochemistry

Background:

  • High throughput analysis is crucial in modern research.
  • Traditional Liquid Chromatography-Mass Spectrometry (LC-MS) methods can be time-consuming.
  • Faster analytical techniques are needed to meet increasing sample demands.

Purpose of the Study:

  • To demonstrate the efficiency of combining high-speed autosampler technology with ultrafast chromatographic separations.
  • To evaluate the performance of Multiple Injection in a Single Experimental Run (MISER) HPLC-MS for microplate analysis.
  • To explore the potential for achieving spectrophotometric plate reader speeds in LC-MS.

Main Methods:

  • Utilized a high-speed autosampler with an injection cycle time of 10.6 seconds.
  • Employed ultrafast chromatographic separations, with some in the sub-5-second range.
Keywords:
Enantiopurity analysisHigh speed autosamplerHigh throughput analysisMultiple injections in a single experimental runUltrafast chiral separations

Related Experiment Videos

  • Performed analysis of 96-well microplates using MISER HPLC-MS.

    • Main Results:

    • Achieved a complete 96-well microplate analysis in only 17 minutes.
    • Demonstrated the feasibility of high throughput analysis with reduced run times.
    • Highlighted the need for even faster autosamplers to complement sub-5-second separations.

    Conclusions:

    • The integration of high-speed autosampler technology and ultrafast chromatography dramatically accelerates high throughput analysis.
    • MISER HPLC-MS offers a significant speed improvement for microplate analysis.
    • Future advancements in autosampler hardware are necessary to further enhance LC-MS analysis speeds, potentially matching spectrophotometric plate readers.