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Potent bactericidal activity of silver nanoparticles synthesized from Cassia fistula fruit
Muhammad Imtiaz Rashid1, Liyakat Hamid Mujawar2, Mohammad Ibrahim Mujallid3
1Center of Excellence in Environmental Studies, King Abdulaziz University, PO Box 80216, Jeddah 21589, Saudi Arabia; Department of Environmental Sciences, COMSATS Institute of Information Technology, 61100 Vehari, Pakistan.
Abstract:
We demonstrated one-step synthesis of silver nanoparticles (AgNPs) from Cassia fistula fruit extract and their antibacterial activity against E. coli and K. pneumoniae. Biogenic AgNPs were characterized by scanning electron microscopy, X-Ray diffraction and fourier transform infrared spectroscopy. Results confirmed spherical shaped AgNPs with an average crystallite size of ∼69 nm. Dose-dependent (0, 10, 20, 40 and 80 μg mL-1) growth kinetic studies showed 100% potency against E. coli (20 μg mL-1) and K. pneumoniae (80 μg mL-1) after 1 and 5 h, respectively. Surface morphology analysis revealed formation of groove/pits in the lysed cell membrane that eventually led to bacterial death.