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Virus-vector relationship in the Citrus leprosis pathosystem
Aline Daniele Tassi1, Laura Cristina Garita-Salazar1, Lilian Amorim1
1Departamento de Fitopatologia e Nematologia, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, CP 9, Piracicaba, SP, 13418-900, Brazil.
Experimental & Applied Acarology
|April 19, 2017
Summary
Citrus leprosis virus C (CiLV-C) is transmitted by Brevipalpus yothersi mites. This study details key aspects of this virus-vector relationship, including acquisition, inoculation, and retention periods, crucial for understanding citrus disease spread.
Area of Science:
- Plant Pathology
- Virology
- Entomology
- Acarology
Background:
- Citrus leprosis is a major citrus disease in the Americas, caused by Citrus leprosis virus C (CiLV-C).
- CiLV-C belongs to the new genus Cilevirus and is transmitted by the mite Brevipalpus yothersi.
- Understanding the virus-vector relationship is critical for managing citrus leprosis epidemiology.
Purpose of the Study:
- To elucidate key components of the CiLV-C/B. yothersi virus-vector relationship.
- To determine virus acquisition, inoculation, and retention parameters.
- To characterize the transmission efficiency and patterns of B. yothersi.
Main Methods:
- Experiments utilized common bean (Phaseolus vulgaris) as a test plant.
- Determined virus acquisition access period (4h), inoculation access period (2h), and latent period (7h).
- Assessed virus retention by individual mites (≥12 days) and transmission rates across all mite developmental stages.
Main Results:
- All developmental stages of B. yothersi transmitted CiLV-C; transovarial transmission was not observed.
- Viruliferous mite efficiency ranged from 25-60%; mites acquired virus from lesions on leaves, fruits, and stems.
- Mites exhibited restricted feeding patterns, typically near leaf veins, indicating localized transmission.
Conclusions:
- The short latent and transmission periods suggest a persistent circulative transmission mode for CiLV-C by B. yothersi.
- Findings provide crucial insights into the epidemiology of citrus leprosis, aiding disease management strategies.
- Further research into the molecular mechanisms of this persistent virus-vector interaction is warranted.