Related Experiment Videos
Probing the Complex Architecture of Multimodular Carbohydrate-Active Enzymes Using a Combination of Small Angle X-Ray
Mirjam Czjzek1, Elizabeth Ficko-Blean2
1UPMC Univ Paris 06, CNRS, UMR 8227, Integrative Biology of Marine Models, Sorbonne Universite, Station Biologique de Roscoff, Place Georges Teissier, 29688, Roscoff, Bretagne, France. czjzek@sb-roscoff.fr.
Methods in Molecular Biology (Clifton, N.J.)
|April 19, 2017
Abstract:
The various modules in multimodular carbohydrate-active enzymes (CAZymes) may function in catalysis, carbohydrate binding, protein-protein interactions or as linkers. Here, we describe how combining the biophysical techniques of Small Angle X-ray Scattering (SAXS) and macromolecular X-ray crystallography (XRC) provides a powerful tool for examination into questions related to overall structural organization of ultra multimodular CAZymes.