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Is cyclic GMP a clinically useful marker for ANF action?

J M Heim1, K Gottmann, J Weil

  • 1Medizinische Klinik Innenstadt, Universität München.

Zeitschrift Fur Kardiologie
|January 1, 1988
PubMed
Summary

Cyclic GMP (cGMP) is a sensitive marker for Atrial Natriuretic Factor (ANF) action. Measuring cGMP in plasma or urine offers a valuable alternative to ANF measurement in clinical settings.

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Area of Science:

  • Biochemistry
  • Physiology
  • Endocrinology

Background:

  • Atrial Natriuretic Factor (ANF) plays a crucial role in cardiovascular regulation.
  • ANF's mechanism of action involves the activation of guanylate cyclase.
  • Understanding ANF's signaling pathway is vital for clinical diagnostics.

Purpose of the Study:

  • To investigate the role of cyclic GMP (cGMP) as a biomarker for ANF activity.
  • To compare the specificity of cGMP elevation in response to particulate vs. soluble guanylate cyclase activators.
  • To evaluate the clinical utility of cGMP measurements as an alternative to ANF assays.

Main Methods:

  • Measurement of plasma and urinary cyclic GMP (cGMP) levels.
  • Administration of ANF and soluble guanylate cyclase activators (e.g., SIN 1).
  • Analysis of cGMP stability in blood samples with EDTA.

Main Results:

  • Plasma cGMP levels significantly increase with ANF, indicating particulate guanylate cyclase activation.
  • Soluble guanylate cyclase activators cause only a modest, non-significant increase in plasma cGMP.
  • Urinary cGMP excretion sensitively reflects plasma ANF levels, unlike urinary ANF excretion.
  • cGMP is stable in EDTA-treated blood, allowing for reliable plasma measurements.

Conclusions:

  • Elevated plasma and urinary cGMP levels are specific indicators of particulate guanylate cyclase activation by ANF.
  • cGMP measurement presents a viable and sensitive alternative for ANF assessment in clinical practice.
  • Urinary cGMP measurement is particularly useful when blood collection is challenging, such as in neonates.

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