Related Experiment Videos
[Renal morphological changes in experimental oxalate nephrolithiasis].
Yu G Motin1, A V Lepilov2, P M Larionov3
1Territorial Clinical Hospital, Barnaul, Altai Territory, Russia.
Arkhiv Patologii
|April 19, 2017
Summary
Early kidney stone formation involves specific cellular changes in tubule and collecting duct epithelium. These changes disrupt cellular balance and activate endoplasmic reticulum stress, potentially worsening stone development.
Area of Science:
- Nephrology
- Cell Biology
- Pathology
Background:
- Oxalate nephrolithiasis is a significant health concern.
- Understanding early renal morphological changes is crucial for intervention.
Purpose of the Study:
- To investigate early renal morphological alterations in lithogenesis.
- To assess the impact of oxalate-chelating compounds on these changes.
Main Methods:
- Experimental oxalate nephrolithiasis model in Wistar rats.
- Morphological and ultrastructural analysis of kidney tissues.
- Immunohistochemical detection of endoplasmic reticulum stress markers.
Main Results:
- Early lithogenesis shows histotopographic changes in renal tubules and collecting ducts.
- Ultrastructural alterations include endoplasmic reticulum expansion and mitochondrial damage.
- Endoplasmic reticulum stress, marked by GADD153 activation, was observed, impairing tubular epithelium.
Conclusions:
- Stereotypic ultrastructural and histotopographic changes occur in early lithogenesis.
- These changes promote stone formation and disrupt cellular homeostasis.
- Endoplasmic reticulum stress activation is implicated in impaired protein synthesis and lithogenesis progression.