An efficient method for gene knock-down by RNA interference in human skin mast cells
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Updated: Nov 6, 2026

Optimized Protocol for Efficient Transfection of Dendritic Cells without Cell Maturation
Published on: July 8, 2011
Tarek Hazzan1, Sven Guhl1, Metin Artuc1
1Department of Dermatology and Allergy, Charité Universitätsmedizin Berlin, Berlin, Germany.
Abstract:
Mast cells (MCs) from human skin have been notoriously resistant to gene manipulation, and a method to knock-down gene expression in in situ differentiated MCs is highly desired. The Dharmacon Accell® transfection system proved successful on several "difficult-to-transfect" cells. In the present work, we therefore tested this method on skin-derived MCs using different siRNA entities. The siRNA was readily taken up, followed by pronounced, specific reduction of gene and protein expression. Hence, we present the first efficient technique for the manipulation of gene expression in primary skin MCs ex vivo, which combines high transfection rates with retained cell viability.
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