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Long Noncoding RNA MEG3 Negatively Regulates Proliferation and Angiogenesis in Vascular Endothelial Cells
Chao He1, Wei Yang1, Jun Yang1
1Institute of Cardiology, China Three Gorges University , Hubei Province, People's Republic of China .
Abstract:
Long noncoding RNA (lncRNA) MEG3 is an important tumor suppressor in several types of human cancers. However, the biological function and underlying mechanism of MEG3 in vascular endothelial cells (VECs) remain unknown. In the present study, we demonstrated the functional importance of lncRNA MEG3 in proliferation and angiogenesis of VECs. MEG3 overexpression significantly suppressed the proliferation and in vitro angiogenesis in VECs, whereas knockdown of MEG3 had the opposite effect. Furthermore, we found that MEG3 exerts its function through negatively regulating miR-9 by acting as a microRNA sponge. Taken together, MEG3-miR-9 plays an important role in proliferation and angiogenesis in VECs.
Insights
Long noncoding RNA (lncRNA) MEG3 suppresses vascular endothelial cell (VEC) proliferation and angiogenesis. MEG3 functions by sponging miR-9, highlighting a novel regulatory mechanism in VECs.
Area of Science:
- Molecular Biology
- Cell Biology
- Oncology
Background:
- Long noncoding RNA (lncRNA) MEG3 is a known tumor suppressor.
- The role of MEG3 in vascular endothelial cells (VECs) is not well understood.
Purpose of the Study:
- To investigate the function and mechanism of lncRNA MEG3 in VECs.
- To explore the involvement of MEG3 in VEC proliferation and angiogenesis.
Main Methods:
- Overexpression and knockdown of MEG3 in VECs.
- Assessment of VEC proliferation and in vitro angiogenesis.
- Analysis of the interaction between MEG3 and miR-9 using microRNA sponge assays.
Main Results:
- MEG3 overexpression inhibited VEC proliferation and angiogenesis.
- MEG3 knockdown promoted VEC proliferation and angiogenesis.
- MEG3 negatively regulated miR-9 activity by acting as a microRNA sponge.
Conclusions:
- lncRNA MEG3 plays a critical role in regulating VEC proliferation and angiogenesis.
- The MEG3-miR-9 axis is a key pathway influencing VEC biological functions.
- MEG3 acts as a tumor suppressor in VECs through miR-9 regulation.