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Laboratory x-ray micro-computed tomography: a user guideline for biological samples
Anton du Plessis1,2, Chris Broeckhoven3, Anina Guelpa1
1CT Scanner Facility, Central Analytical Facilities, Stellenbosch University, Stellenbosch, 7602, South Africa.
Gigascience
|April 19, 2017
Summary
This guide offers a how-to for laboratory x-ray micro-computed tomography (micro-CT), detailing project planning, setup, scanning, and reconstruction for new users. It aims to promote micro-CT adoption in scientific research by familiarizing users with its concepts and applications.
Area of Science:
- Scientific Imaging
- Morphological Analysis
- Biological Research
Background:
- Laboratory x-ray micro-computed tomography (micro-CT) is increasingly utilized for non-destructive imaging.
- It enables detailed visualization of morphological structures in various scientific fields.
- New researchers often require guidance for effective implementation.
Purpose of the Study:
- To provide a practical "how-to" guide for new users of micro-CT.
- To outline essential steps for planning and executing micro-CT research projects.
- To promote broader adoption of micro-CT in scientific studies.
Main Methods:
- Detailed explanation of micro-CT setup and scanning parameters.
- Guidance on image reconstruction and data visualization techniques.
- Use of a Jackson's chameleon specimen as a practical example throughout the guide.
Main Results:
- Demonstration of micro-CT's capability for non-destructive morphological imaging.
- Illustrative examples of scanning at different settings to show parameter impact.
- Successful application of the guide's methodology to a biological specimen.
Conclusions:
- Micro-CT is a powerful tool for non-destructive morphological studies.
- This guide simplifies the process for new users, encouraging wider application.
- Familiarity with micro-CT concepts and applications will drive future scientific research.