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Chapter 6 Technology of Aseptic Cryoprotectant-Free Vitrification of Human ICSI Spermatozoa
Vladimir Isachenko1, Raul Sanchez2, Peter Mallmann3
1Department of Obsterics and Gynecology, Medical Faculty, University Maternal Hospital, Cologne University, Kerpener Str 34, 50931, Cologne, Germany. vladimir.isachenko@googlemail.com.
Methods in Molecular Biology (Clifton, N.J.)
|April 20, 2017
Abstract:
The aim of this chapter was to describe the standardized aseptic technology of permeable cryoprotectant-free vitrification of human spermatozoa in capillaries (for ICSI or IVF in microvolume). Spermatozoa, vitrified by this technology, are free from seminal plasma owing to swim-up procedure preceding vitrification and are free from permeable cryoprotectants. They are ready for further use immediately after warming without any additional treatment.